Journal: Cell Reports Medicine

Article Title: Tissue-specific transcriptional profiles and heterogeneity of natural killer cells and group 1 innate lymphoid cells

doi: 10.1016/j.xcrm.2022.100812

Figure Lengend Snippet: Inter-tissue-specific signatures of NK and ILC1 subpopulations (A) Transcriptome-based clusters computed for each organ separately, projected onto the multi-organ integrated UMAP obtained with Harmony. (B) Heatmap representing overlap coefficient dissimilarity between transcriptome-based clusters calculated separately for each organ. (C) Dot plot of NK cell-specific versus ILC1-specific markers. Intermediate clusters were not taken into account. (D) Dot plot of specific markers for each cluster. (E) Flow cytometry profiles of CD49a, DNAM-1, PD-1H, and SDC4 expression, analyzed in Lin − NKp46 + NK1.1 + cells. Histograms represent the frequency of CD49a − CD49b + NK cells and CD49a + CD49b − ILC1s for each indicated marker across organs. Data are shown as mean ± SEM and are pooled from two independent experiments. Each point represents a pool of two mice.

Article Snippet: APC anti-mouse SDC4 (REA640) , Miltenyi Biotec , Cat# 130-109-831; RRID: AB_2653641.

Techniques: Flow Cytometry, Expressing, Marker

Journal: Cell Reports Medicine

Article Title: Tissue-specific transcriptional profiles and heterogeneity of natural killer cells and group 1 innate lymphoid cells

doi: 10.1016/j.xcrm.2022.100812

Figure Lengend Snippet:

Article Snippet: APC anti-mouse SDC4 (REA640) , Miltenyi Biotec , Cat# 130-109-831; RRID: AB_2653641.

Techniques: Recombinant, Saline, Staining, Cell Isolation, Sensitive Assay, Software

Journal: Brain Pathology

Article Title: Overexpression of the ubiquitin‐editing enzyme A20 in the brain lesions of Multiple Sclerosis patients: moving from systemic to central nervous system inflammation

doi: 10.1111/bpa.12906

Figure Lengend Snippet: List of antibodies used for neuropathological characterization of human post‐mortem snap‐frozen brain tissue by Immunohistochemistry (IHC) and immunofluorescence (IF). Abbreviations: MOG = myelin‐oligodendrocyte glycoprotein; MHC II = major histocompatibility complex class II molecules, GFAP = glial fibrillary acidic protein; NEFH = neurofilament heavy chain; PFA = paraformaldehyde.

Article Snippet: CD68 , Mouse , Monoclonal , 514H12 , IF 1:50 , 4% PFA , MCA1851T , AbD Serotec , macrophages.

Techniques: Immunohistochemistry, Immunofluorescence, Immunopeptidomics, Marker

Journal: International Journal of Ophthalmology

Article Title: Promotion of axon regeneration and inhibition of astrocyte activation by alpha A-crystallin on crushed optic nerve

doi: 10.18240/ijo.2016.07.04

Figure Lengend Snippet: A: Longitudinal sections through the adult mouse optic nerve showing the CSPG levels (green) in the ONC injury (injury only) (a-c, and c zoomed image d), PBS-treated ONC injury group (PBS injection) (e-g, and g zoomed image h) and αA-crystallin-treated ONC injury groups (α-A injection) (i-k, and k zoomed image l). B, C: Western blots showing that the expression of CSPGs was obviously increased in the injury only and PBS-treated groups. However, in the αA-crystallin-treated group, the CSPGs were significantly decreased compared to the injury and PBS-treated (P<0.05) groups. cr: The middle of crush site. The arrows indicate CSPG-positive labeling. The data were shown as means±SD (n=5). aP<0.05.

Article Snippet: The sections were then incubated with the following primary antibodies in 3% goat serum overnight at 4°C: rabbit anti-glial fibrillary acidic protein (GFAP) (Abcam, Cambrige, MA, USA, 1:500), mouse anti-beta Tubulin (TUJ1) monoclonal antibody (Earthox, San Francisco, USA, 1:300), mouse anti-feline CSPG Brain core protein (US Biological, US, 1:400), and mouse anti-neurocan antibody (EMD Millipore, 1:500).

Techniques: Injection, Western Blot, Expressing, Labeling